The Procrea Andrology Laboratory has the infrastructure to carry out the necessary procedures for the proper handling of the semen samples, always applying security measures and best quality in strict adherence to the necessary measures for proper preservation.
Among the services offered by the Andrology Laboratory there are:
- Semen cultures
- Sperm training
- DNA fragmentation
- Testicular biopsy analysis
- Sperm Bank
- Donor Sperm Bank
- Sperm freezing
Three main factors are analyzed and evaluated in semen: Sperm concentration (how many there are), mobility (how much they move) and morphology (how well trained are).
Microbiological study that detects the presence and growth of bacteria and fungi in the semen. The main microorganisms that are detected are: Mycoplasma genitalium, Ureaplasma urealyticum and Chlamydia trachomatis.
Spermatozoids are separated from the seminal fluid and are prepared in the laboratory to simulate the process that would naturally occur within the uterus. The techniques we use are: Separation by density gradients and swim-up, in this way we get the best spermatozoids for assisted reproduction procedures.
To assess whether the genetic material of the sperm is full or have breaks that compromise the normal development of the embryo. The more injuries have sperm, lower its ability to fertilize an ovule.
Testicular biopsy analysis
It involves removing a small piece of testicular tissue and check it under the microscope to check for sperm. This technique is used when no sperm in the ejaculate obtained.
This service is mainly offered to patients who for some reason can not present the day you should fertilize eggs from their partners, which are close to a vasectomy or starting a cancer treatment; thus we retain samples for later use.
Donor Sperm Bank
We have a catalog of young donors who are subjected to rigorous studies. These consist of psychological evaluations, genetic studies and laboratory tests such as:
- Hepatitis B and C
- HIV (AIDS)
When requesting a catalog you can meet the physical characteristics of the donor, their siblings, parents and grandparents; and if he has an hereditary disease.
Semen samples are mixed with a cryoprotectant that prevents damage to sperm and then keep them in tanks with liquid nitrogen at -196 ° C, so we can preserve samples long term.
LABORATORY IN-VITRO FERTILIZATION
Low complexity Procedures
Sexual relations with the couple are scheduled to match with ovulation of the patient, either with ovarian stimulation or naturally, in the days most likely to achieve fertilization.
It involves making a seminal training and with the aid of a sterile cannula, deposit them in the uterus. The patient is subject to a process of hormonal stimulation but not ovarian puncture is performed.
High complexity Procedures
It is a procedure performed under general anesthesia and involves puncturing the ovaries using a transvaginal ultrasound and a needle with which the follicles are aspirated. Each follicle contains 1 oocyte that will be recovered, washed and incubated for subsequent fertilization.
Acronym for In-Vitro Fertilization. It consists in ovarian stimulation, ovarian puncture to retrieve the ovules and spermatozoa capacitation and then incubating them with oocytes in order that fertilization occurs.
An acronym for Intracytoplasmic spermatzoid injection. The procedure is very similar to IVF but instead of just putting the gametes in the same medium, it is injected into each ovule with a spermatozoid.
Acronym in English for Physiological Intracytoplasmic Sperm Injection. Consists in using a plate having a substance to which the best spermatzoids adhere, which are then injected into oocytes. It is recommended when sperm motility is low.
Once retrieved oocytes, mature ones are selected and undergo a process called vitrification. This process consists to replace the intracellular water by other sustance not able to form crystals upon freezing and allows us to keep them in liquid nitrogen at -196 ° C for future use. Oocytes can be preserved for more than 10 years without suffering any damage.
Egg donors are healthy and young women who anonymously, voluntary and altruistic, agree to donate their gametes for couples who require it. They are subjected to rigorous studies consisting of psychological evaluations, genetic studies and laboratory tests such as:
- Hepatitis B and C
- HIV (AIDS)
- • Vaginal culture
Acronym for Preimplantation Genetic Diagnosis. It consists of a chromosomal evaluation of the embryos to detect anomalies and to be able to choose healthy embryos for the transfer. The chromosomes studied are 13,15, 16,18, 21,22, X and Y, with which we discard the most important anomalies and we also know the sex of the embryo. To perform this technique, it is necessary to perform a biopsy, which consists of removing a cell from the embryo in order to analyze it.